The protocol uses a single primary antibody with nuclear counterstaining, but it can be extended to double antibody staining. It requires an inverted microscope with fluorescence illumination and long working distance objectives.
Wednesday,Jul 15,2009 Source: Aplin Laboratory, Reproductivehttp://faculty.washington.edu/rhevner/Slice_Culture.doc
Wednesday,Jul 15,2009 Source: Robert F. Hevner Lab, Universihttp://www.protocol-online.org/prot/Protocols/Freezing-and-Thawing-of-MEFs-3462.html
Wednesday,Jul 15,2009 Source: This protocol is very useful tThis method enables the researcher to explant terminal placental villi onto a three dimensional matrix of either collagen or Matrigel and monitor the formation of anchoring cytotrophoblast columns. The resulting cells can be stimulated to assume a migratory phenotype by growth factors. TGFb1 inhibits outgrowth.
Wednesday,Jul 15,2009 Source: Aplin Laboratory, ReproductiveThis method is used to study secretion of proteins and prostaglandins by endometrium from the cow, ewe, mare, bitch and other species. The technique is also useful for culture of peri-implantation conceptuses and placental tissues for metabolic labelling studies and to obtain conceptus secretory proteins for biological studies.
Wednesday,Jul 15,2009 Source: P.J. Hansen Labhttp://www.protocol-online.org/cgi-bin/prot/jump.cgi?ID=61
Wednesday,Jul 15,2009 Source: Dr. William H. Heidcamp, BioloMethod for creating cell lines from tumor tissue
Wednesday,Jul 15,2009 Source: Cowin Lab, New York University
