shRNA Explorer
Very nice tool for designing oligos to construct shRNA vectors.
Sunday,Jul 12,2009 Source: Gene LinkshRNA Design Tool
This tool will generate hairpin siRNA-encoding DNA oligonucleotide insert sequences from an input siRNA target sequence, for cloning into a pSilencer siRNA Expression Vector. The program will add the loop sequence and overhangs for cloning. It is also helpful for design shRNA for other cloning vectors.
Sunday,Jul 12,2009 Source: AmbionReal-Timer PCR Primer Database
A frequently updated database of validated primer/probe sets for quantitation of a large number of genes of various organisams.
Sunday,Jul 12,2009 Source: Steven W. Johnson, Ph.DPrimer Bank
PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). There are several ways to search for primers: by GenBank Accession, NCBI protein accession, LocusLink ID, PrimerBank ID or Keyword (gene description). PrimerBank contains about 180,000 primers covering most known human and mouse genes.
Sunday,Jul 12,2009 Source: Dr. Xiaowei Wang at the DepartReal-time PCR (TaqMan) Primer Design
This online tool designs real-time PCR (TaqMan) primers for you. It accepts an accession number or a sequence as input
Sunday,Jul 12,2009 Source: GenScriptReal-Time PCR (qPCR) Primer Design Tool
You can input a sequence accession number or a sequence, the program will return qPCR primers
Sunday,Jul 12,2009 Source: IDTHYDEN (Downloadable Program)
HYDEN (HighlY DEgeNerate primers) is a program for designing pairs of degenerate primers for a given set of DNA sequences. HYDEN works well for large input sets of genomic sequences (e.g., hundreds of sequences of length 1Kbp). HYDEN is a batch (i.e., command-line, as opposed to graphical interface) program, available for Windows XP (downloadable version) and Linux (upon request).
Sunday,Jul 12,2009 Source: Chaim Linhart and Ron Shamir,CODEHOP -COnsensus-DEgenerate Hybrid Oligonucleotide Primers
PCR primers designed from protein multiple sequence alignments
Sunday,Jul 12,2009 Source: Blocks WWW ServerAntisense RNA (aRNA) Design
Free online tool for antisense RNA design.
Sunday,Jul 12,2009 Source: IDTSirna - SiRNA Design
Sfold aims to provide tools for improved siRNA design by combining secondary structure and accessibility prediction with empirical design rules. Free.
Sunday,Jul 12,2009 Source: Wadsworth Bioinformatics Cente方法搜索
输入关键字搜索方法
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