Western Blot with Platelet Protein
Detailed western protocol devoted to detecting paltelet protein but can also be used for general purpose.
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisThrombophagocytosis
This protocol represents an Ab-mediated thrombophagocytosis modification. Well written with illustration.
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisSerum Separation from Whole Blood
Method for obtaining serum from clotted blood
Tuesday,Jul 14,2009 Source: Dr. Simon Dawson, Department oPlatelet-associated Ig (PAIg) Elution
Platelet-associated Ig (PAIg) could be eluted from the platelets. As a result of this procedure platelets get destroyed and the eluted Ig is formed of platelet surface-bound Ig and , mainly, of internal pools of Ig (from a-granules). That is why the caution must be taken when interpreting results.
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisPlatelet Preparation
This protocol is based on differential centrifugation that allows to separate platelets from blood cells.
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisErythrophagocytosis
The erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisELISA with Platelet
This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg)
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisCytology of Mammalian Blood
preparation of blood smear and microscopic examination of whole blood for identification of formed elements
Tuesday,Jul 14,2009 Source: David R. Caprette, Rice UniverCoating of Platelets with Antibody in vitro
Antibody-coated platelets (opsnized) may be used in the subsequent thrombophagocytosis assay.
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisCMFDA Labeling of Platelet
CMFDA (5-chloromethylfluorescein diacetate) is a lipophilic tracer that has an enormous advantage over ordinary tracers (e.g. FITC) because it can be introduced into live (!) cells. Once inside the cell this dye undergo some covalent changes restricting from its passgae to extracellular milieu
Tuesday,Jul 14,2009 Source: Viral Immunity & Pathogenesis方法搜索
输入关键字搜索方法
Loading
