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    切除大鼠双侧卵巢方法

    一.术前准备 1.备四个尿杯,一个换药碗 1个尿杯装活力碘 1个尿杯装75%医用酒精 1个尿杯装生理盐水 1个尿杯装无菌棉签 1个换药碗装无菌纱布和棉球 2.配制麻醉药(10%水合氯醛液) 需要一

    Thursday,Jul 30,2009        Source: Biolab

    Western Blot with Platelet Protein

    Detailed western protocol devoted to detecting paltelet protein but can also be used for general purpose.

    Tuesday,Jul 14,2009        Source: Viral Immunity & Pathogenesis

    Thrombophagocytosis

    This protocol represents an Ab-mediated thrombophagocytosis modification. Well written with illustration.

    Tuesday,Jul 14,2009        Source: Viral Immunity & Pathogenesis

    Serum Separation from Whole Blood

    Method for obtaining serum from clotted blood

    Tuesday,Jul 14,2009        Source: Dr. Simon Dawson, Department o

    Platelet-associated Ig (PAIg) Elution

    Platelet-associated Ig (PAIg) could be eluted from the platelets. As a result of this procedure platelets get destroyed and the eluted Ig is formed of platelet surface-bound Ig and , mainly, of internal pools of Ig (from a-granules). That is why the caution must be taken when interpreting results.

    Tuesday,Jul 14,2009        Source: Viral Immunity & Pathogenesis

    Platelet Preparation

    This protocol is based on differential centrifugation that allows to separate platelets from blood cells.

    Tuesday,Jul 14,2009        Source: Viral Immunity & Pathogenesis

    Erythrophagocytosis

    The erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts

    Tuesday,Jul 14,2009        Source: Viral Immunity & Pathogenesis

    ELISA with Platelet

    This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg)

    Tuesday,Jul 14,2009        Source: Viral Immunity & Pathogenesis

    Cytology of Mammalian Blood

    preparation of blood smear and microscopic examination of whole blood for identification of formed elements

    Tuesday,Jul 14,2009        Source: David R. Caprette, Rice Univer

    Coating of Platelets with Antibody in vitro

    Antibody-coated platelets (opsnized) may be used in the subsequent thrombophagocytosis assay.

    Tuesday,Jul 14,2009        Source: Viral Immunity & Pathogenesis
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