切除大鼠双侧卵巢方法
一.术前准备 1.备四个尿杯,一个换药碗 1个尿杯装活力碘 1个尿杯装75%医用酒精 1个尿杯装生理盐水 1个尿杯装无菌棉签 1个换药碗装无菌纱布和棉球 2.配制麻醉药(10%水合氯醛液) 需要一
Thursday,Jul 30,2009 Source: Biolab一.术前准备 1.备四个尿杯,一个换药碗 1个尿杯装活力碘 1个尿杯装75%医用酒精 1个尿杯装生理盐水 1个尿杯装无菌棉签 1个换药碗装无菌纱布和棉球 2.配制麻醉药(10%水合氯醛液) 需要一
Thursday,Jul 30,2009 Source: BiolabDetailed western protocol devoted to detecting paltelet protein but can also be used for general purpose.
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisThis protocol represents an Ab-mediated thrombophagocytosis modification. Well written with illustration.
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisMethod for obtaining serum from clotted blood
Tuesday,Jul 14,2009 Source: Dr. Simon Dawson, Department oPlatelet-associated Ig (PAIg) could be eluted from the platelets. As a result of this procedure platelets get destroyed and the eluted Ig is formed of platelet surface-bound Ig and , mainly, of internal pools of Ig (from a-granules). That is why the caution must be taken when interpreting results.
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisThis protocol is based on differential centrifugation that allows to separate platelets from blood cells.
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisThe erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts
Tuesday,Jul 14,2009 Source: Viral Immunity & PathogenesisThis modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg)
Tuesday,Jul 14,2009 Source: Viral Immunity & Pathogenesispreparation of blood smear and microscopic examination of whole blood for identification of formed elements
Tuesday,Jul 14,2009 Source: David R. Caprette, Rice UniverAntibody-coated platelets (opsnized) may be used in the subsequent thrombophagocytosis assay.
Tuesday,Jul 14,2009 Source: Viral Immunity & Pathogenesis