转化子的筛选与检测
用于基因工程的许多质粒载体含有编码抗生素抗性的基因，如 pBR322 携带有氨苄青霉素抗性基因（ amp ）和四环素抗性基因（ tet ）。这些基因作为载体的标记，其中一个（如 amp ）可以
Thursday,Aug 06,2009 Source: BiolabStandard (alkaline lysis) Mini-Prep
Standard protocol for mini-prep and recipe for solution I, II and III.
Thursday,Jul 23,2009 Source: Goldberg LabSmall-Scale Purification of Plasmid
Detailed procedures with recipes.
Thursday,Jul 23,2009 Source: Hideaki Shiraishi, Kyoto UniveQuick Plasmid DNA Preparation
This protocol gives very clean plasmid preps for restriction digests and cloning. However, due to the alkaline lysis step, the DNA is often nicked and may not give exceptional sequence data.
Thursday,Jul 23,2009 Source: Mike A DyerQuick DNA Plasmid Prep. : Boiling 10 minute protocol
This is a very fast mini-prep protocol which is suitable for sequence analysis and restriction digests. Although the yield is higher, there is considerable chromosomal DNA, RNA and protein contamination.
Thursday,Jul 23,2009 Source: Mike A DyerPreparing Overnight Bacteria Culture
This is a basic procedure for high school students and useful for those who are new to molecular biology.
Thursday,Jul 23,2009 Source: LaboratoryExperiments.comPlasmid Quickpreps
Nice protoocl for preparing plasmid DNA from a single colony
Thursday,Jul 23,2009 Source: Kay Schneitz LabPlasmid Minipreps
http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=1691
Thursday,Jul 23,2009 Source: Bowtell LabPlasmid Miniprep
Mini-Prep procedure is used to isolate small plasmid DNA from bacteria while limiting contaminating proteins and genomic DNA. The plasmid quality is acceptable for restriction analysis, sequencing, cloning, or other purposes, but should not be used with out additional cleanup for embryonic injections.
Thursday,Jul 23,2009 Source: Hackett Lab, University of MinPlasmid MiniPrep
Minipeps without overnight culture (8 h from colony to plasmid DNA). This method uses Qiagen solutions.
Thursday,Jul 23,2009 Source: Methods.info方法搜索
输入关键字搜索方法
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