病毒

进入实验方法社区讨论

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Titration of Retrovirus

http://www.methodbook.net/virus/titratn.html

Sunday,Jul 19,2009 Source: Matt Lewis, Department of Path

Retroviral infection of primary human keratinocytes (PHKs)

PHKs must be actively dividing to be successfully infected PHKs must never become confluent because they become difficult to trypsinize

Sunday,Jul 19,2009 Source: Department of Pathology Univers

Generation of recombinant baculoviruses by co-transfection

For co-transfection prepare at least 10mg of highly purified plasmid DNA. Spodoptera frugiperda cells are sensitive to some contaminants found in crude plasmid preparations, which cannot be removed by phenol/chloroform extraction or ethanol precipitation.

Sunday,Jul 19,2009 Source: Bjorkman Group, Howard Hughes