Titration of Retrovirus
http://www.methodbook.net/virus/titratn.html
Sunday,Jul 19,2009 Source: Matt Lewis, Department of PathRetroviral infection of primary human keratinocytes (PHKs)
PHKs must be actively dividing to be successfully infected PHKs must never become confluent because they become difficult to trypsinize
Sunday,Jul 19,2009 Source: Department of Pathology UniversGeneration of recombinant baculoviruses by co-transfection
For co-transfection prepare at least 10mg of highly purified plasmid DNA. Spodoptera frugiperda cells are sensitive to some contaminants found in crude plasmid preparations, which cannot be removed by phenol/chloroform extraction or ethanol precipitation.
Sunday,Jul 19,2009 Source: Bjorkman Group, Howard HughesCreation and Use of Infectious Virus Vector
http://www.stewartlab.net/Protocols/Making_Virus_1.htm
Sunday,Jul 19,2009 Source: Stewart Lab, Washington UniverTotal DNA Isolation from Filamentous Fungi
Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chloride, or suitable only for small scale preparations. This protocol describes a simple method for total DNA preparation, yielding a product of quality suitable for restriction digestion and library construction.
Sunday,Jul 19,2009 Source: Fungal Genetics Stock CenterSmall-scale DNA prep from Neurospora
Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an average of 0.6 micrograms of genomic DNA that is suitable for Southern analysis or PCR
Sunday,Jul 19,2009 Source: Fungal Genetics Stock CenterRNA Miniprep from Neurospora
Procedures of RNA extraction published so far follow roughly three different approaches, where phenol/chloroform, guanidinium salts, and/or LiCl is used. However, these protocols have some disadvantages. This method, combining the advantages of phenol extraction and LiCl precipitation, is for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities.
Sunday,Jul 19,2009 Source: Fungal Genetics Stock CenterQuick RNA Miniprep from Neurospora(ATA)
The method involves the use of a triphenylmethane dye, aurintricarboxylic (ATA), to protect the RNA
Sunday,Jul 19,2009 Source: Fungal Genetics Stock CenterGrowing Fungi for DNA Extraction
Use of reverse agar for cultivation of fungi for DNA extraction
Sunday,Jul 19,2009 Source: Fungal Genetics Stock CenterFast DNA extraction from Neurospora
A simple method for growing Neurospora and for isolation of DNA that may be performed in two days from start to finish. The growth of mycelia in Petri plates eliminates the need for large numbers of flasks when growing many cultures for DNA isolation
Sunday,Jul 19,2009 Source: Fungal Genetics Stock Center方法搜索
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