Purification of human mononuclear cells and neutrophils
cell isolation
Saturday,Jul 18,2009 Source: Springer Lab, Harvard UniversiPreparation of Lymphocyte Cell Pellet for Storage
Following propagation to 1 X 108 cells, lymphoblastoid cells are conveniently stored at -80 degrees C to preserve the high molecular weight DNA in the cells until the DNA is purified. This procedure describes the steps required to harvest and freeze the cells for long term storage.
Saturday,Jul 18,2009 Source: Donis-Keller labPreparation of Lymphoblastoid Cell Lines for Long-Term Storage
Cell are frozen in RPMI-1640 with 15% Fetal Bovine Serum + 10% DMSO. Cultures are frozen slowly using a Model 700 Controller freezing chamber...
Saturday,Jul 18,2009 Source: Donis-Keller labPhagocytosis
http://www.hopkinsmedicine.org/cellbio/devreotes/phagocytosis.htm
Saturday,Jul 18,2009 Source: Devreotes LabPeritoneal Macrophage Preparation
This well-written yet simple protocol is to prepare peritoneal macrophage cell for subsequent thrombophagocytosis test.
Saturday,Jul 18,2009 Source: Viral Immunity & PathogenesisNeedle Assay for Chemotaxis
http://www.hopkinsmedicine.org/cellbio/devreotes/needle.htm
Saturday,Jul 18,2009 Source: Devreotes LabMonocytes/Macrophages: Enrichment of PBMCs with Monocytes
This protocol is used to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protocol contains 40-70% monocytes. This cell suspension is than used for positive or negative MACS separation.
Saturday,Jul 18,2009 Source: Methods.infoMaintaining Lymphoblastoid Cell Lines
To grow lymphoblastoid cells for permanent storage and for DNA extraction.
Saturday,Jul 18,2009 Source: Donis-Keller labMacrophage Extraction
http://www.genetics.ucla.edu/labs/lusis/macrophageextract.htm
Saturday,Jul 18,2009 Source: Lusis Lab, UCLAMacrophage Depletion by Clodronate
Clodronate (dichloromethylene diphosphonate) is used for treatment of osteolytic diseases and osteoporosis Its effect is due to inhibittion of the function of osteoclasts. This drug is also taken up by macrophages and rapidly causes apoptosis of these cells.
Saturday,Jul 18,2009 Source: Viral Immunity & Pathogenesis方法搜索
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