Double Immunofluorescence stain
Any type of tissue is suitable for this technique, as long as the antigenicity for your antigen(s) is preserved. Use cytocentrifuged cells or frozen or paraffin dewaxed sections
Friday,Jul 17,2009 Source: Giorgio Cattoretti, Columbia UAntibody Cleanup
In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use.
Friday,Jul 17,2009 Source: YGACA Semi-Permenant Mounting Medium for Immunofluorescence Microscopy
A recipe for Moviol, an inexpensive mounting medium that is easy to prepare.
Friday,Jul 17,2009 Source: House Ear Institute方法搜索
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