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免疫细胞化学

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    Procedure for fixing and staining adherent cells for immunofluorescence

    This procedure describes how to process cells for immunofluoresence microscopy.

    Friday,Jul 17,2009        Source: The Science Advisory Board

    Fixation Cells with Glutaraldehyde

    http://www.protocol-online.org/cgi-bin/prot/jump.cgi?ID=2902

    Friday,Jul 17,2009        Source: Yu-li Wang's Lab, University o

    Fixation Cell with Formaldehyde-Acetone Detailed protocol for fixing cells for staining

    Detailed protocol for fixing cells for staining

    Friday,Jul 17,2009        Source: Yu-li Wang's Lab, University o

    Antibleaching Cocktails

    http://www.protocol-online.org/cgi-bin/prot/jump.cgi?ID=2906

    Friday,Jul 17,2009        Source: Yu-li Wang's Lab, University o

    Using Resin Sections for Immunofluorescence

    A protocol for applying antibodies with fluorescent markers onto sections of resin-embedded material. The thin sections help increase resolution.

    Friday,Jul 17,2009        Source: House Ear Institute

    Silver Enhancement of Colloidal Gold

    How to precipitate silver onto small colloidal gold particles so that the signal can be detected by light microscopy.

    Friday,Jul 17,2009        Source: House Ear Institute

    Problems with Immunolabeling

    When labeling cells or sections with antibodies or other affinity markers, the results are not easily interpreted. Look here for some help

    Friday,Jul 17,2009        Source: House Ear Institute

    Preparation of Colloidal Gold Probes

    See how easily, and inexpensively, these expensive probes can be prepared. Excellent information if large volumes of gold probes are required.

    Friday,Jul 17,2009        Source: House Ear Institute

    Introduction to Immunocytochemistry

    A brief overview of common available methods.

    Friday,Jul 17,2009        Source: (House Ear Institute

    Immunostaining of Cells Adherent to Coverslips

    http://www.protocol-online.org/cgi-bin/prot/jump.cgi?ID=447

    Friday,Jul 17,2009        Source: Laurie Lab, University of Virg
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