ELISA

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Sandwich ELISA For Detecting Secreted ICAM-1

http://www.protocol-online.org/cgi-bin/prot/jump.cgi?ID=2348

Friday,Jul 17,2009 Source: Springer Lab, Harvard Universi

Sandwich ELISA

The sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites, capable of binding to antibody, since at least two antibodies act in the sandwich. So

Friday,Jul 17,2009 Source: Kitto Lab, The University of T

Sandwich ELISA Protocol

Sandwich ELISAis used to determine the antigen concentration in unknown samples. This protocol describes both the principals and prcedures of sandwich ELISA, it also lists troubleshootings.

Friday,Jul 17,2009 Source: Chemicon

Indirect ELISA

http://www.sigmaaldrich.com/life-science/cell-biology/antibodies/antibodies-application/protocols/elisa.html

Friday,Jul 17,2009 Source: Sigma

Indirect ELISA

The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones. http://kitto.cm.utexas

Friday,Jul 17,2009 Source: Kitto Lab, The University of T

Indirect ELISA (

For accurate quantitative results, always compare signal of unknown samples against those of a standard curve. Standards (duplicates or triplicates) and blank must be run with each plate to ensure accuracy. http://www.abcam.com/index.html?p

Friday,Jul 17,2009 Source: Abcam