原位杂交in situ hybridization(以斑马鱼为例)
固定 收集斑马鱼的胚胎，在Holfretor水中培养，到达所需要的发育时期时，用蛋白酶去除卵膜，用4%多聚甲醛固定，在4℃保存，二十四小时后用50%甲醇2%多聚甲醛溶液洗，然后换成甲醇，
Thursday,Aug 06,2009 Source: BiolabTissue Preparation for In Situ Hybridization
http://www.emory.edu/WILCOX/tissueprep.html
Monday,Jul 20,2009 Source: Wilcox LabStorage of Chromosome Preparations for FISH
The quality of stored chromosome preparations varies considerably from one case to another. In some cases, good preparations can be obtained from cells stored in fixative, while in other cases, the quality of the chromosome spreads obtained from cell pellets is poor. It is therefore recommended that specimens harvested for chromosome preparations which are to be used for FISH and SKY be stored both as cell pellets and as slide preparations.
Monday,Jul 20,2009 Source: Thomas Ried's Lab, NCISequential RNA and DNA fluorescence in situ hybridization (FISH)
The most efficient way to map gene loci and their activity is sequential RNA and DNA FISH. This is an important technique to uncover how gene positioning is linked to activity.
Monday,Jul 20,2009 Source: Epigenome Network of ExcellencScreening cDNA Clones by RNA In Situ Hybridization
The RNA in situ procedure described below is based on the protocol developed by Tautz and Pfeifle, but adapted to allow the screening of 96 RNA probes on whole-mount Drosophila embryos at the same time.
Monday,Jul 20,2009 Source: Goodman LaboratoryRNA in situ protocol for DNA and RNA probes
http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=306
Monday,Jul 20,2009 Source: Carroll LabProtein-DNA Telomere FISH
http://www.stewartlab.net/Protocols/Telomere_Fish_1.htm
Monday,Jul 20,2009 Source: Stewart Lab, Washington UniverPreparation of Stock Solutions for FISH
Recipes for FISH solutions
Monday,Jul 20,2009 Source: Institute of Pathology,HumboldPreparation of Paraffin Sections and Frozen Tissue for FISH
http://www.riedlab.nci.nih.gov/publications/2412.2845_Paraff_FrozTiss_Slide_Pretreat.pdf
Monday,Jul 20,2009 Source: Thomas Ried's Lab, NCImRNA In Situ Hybridization withNick Translated Probes
http://www.cshl.edu/
Monday,Jul 20,2009 Source: Spector Lab方法搜索
输入关键字搜索方法
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