癌症遗传学

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Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)

PCR Method for mutation detection.

Monday,Jul 20,2009 Source: Reddy research laboratory, Neu

Step by Step SSCP

This protocol is for Single-Strand Conformation Polymorphism (SSCP) analysis. It is basically the same as any SSCP protocol you will find in the literature. The only major difference is that the author recommends doing a two reaction PCR (unless you are screening more than 48 individuals, then you should optimize PCR conditions and amplify hot the first time around). The first reaction is a "normal" cold PCR to amplify the intended products. Check that these reactions were suc

Monday,Jul 20,2009 Source: Travis Glenn, Laboratory of Mo

Serial Analysis of Gene Expression

SAGE is a powerful tool that allows the analysis of overall gene expression patterns with digital analysis. Because SAGE does not require a preexisting clone, it can be used to identify and quantitate new gene as well as know genes.

Monday,Jul 20,2009 Source: SAGE

Loss of Heterozygosity

This method is used to detect genomic DNA deletions in tumor cells

Monday,Jul 20,2009 Source: Molecular Profiling Initiative

Comparative Gene Expression Study Using Microarray

http://www.cs.wustl.edu/~jbuhler//research/array/

Monday,Jul 20,2009 Source: Jeremy Buhler

Clonality: X-chromosome Inactivation

X chromosome inactivation (methylation) can be used to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome. Clonal cell populations will show "loss" of the non-methylated allele after restriction digest. The assay can be performed on DNA recovered from microdissected samples. Both frozen tissue and fixed-embedded archival tissue

Monday,Jul 20,2009 Source: Molecular Profiling Initiative