生物芯片的制作
对于一些实验室来说，如果现成的商品化芯片不能满足研究需要，而自行设计向厂家定做芯片也不能满足时间的需要时，就需要自制芯片。要成功的制作芯片，需要准备 3 大材料：准备
Thursday,Aug 06,2009 Source: Biolab原位杂交in situ hybridization(以斑马鱼为例)
固定 收集斑马鱼的胚胎，在Holfretor水中培养，到达所需要的发育时期时，用蛋白酶去除卵膜，用4%多聚甲醛固定，在4℃保存，二十四小时后用50%甲醇2%多聚甲醛溶液洗，然后换成甲醇，
Thursday,Aug 06,2009 Source: BiolabPreparing Tail Or Toe DNA For Southern Blot Analysis
http://transgenic.cwru.edu/protocols/prep_toe_dna_southerns.html
Monday,Jul 20,2009 Source: Case Transgenic and TargetingNon-organic Isolation of Tail DNA
http://www.jax.org/imr/tail_nonorg.html
Monday,Jul 20,2009 Source: Jackson LabsIsolation of DNA from Mouse Tail Biopsies
http://www.med.umich.edu/tamc/tDNA.html
Monday,Jul 20,2009 Source: University of Michigan TransgeDNA preparation from mouse tails
Avoid Shearing of the DNA by limiting mixing to inversion and gentle shaking. Vortexing of samples is not required to perform the following protocols. Avoid contamination by rigorously cleaning pipetman and using autoclaved tips during manipulation of genomic DNA and reagents to be used with genomic DNA...
Monday,Jul 20,2009 Source: Dr. Ornitz's Lab, Washington UDNA Extraction from Tail Biopsies
Phenol:chloroform extraction method
Monday,Jul 20,2009 Source: Jackson LabsExtraction Of DNA From Mouse Tails
http://stanxterm.aecom.yu.edu/wiki/index.php?page=Tail_DNA_extraction
Monday,Jul 20,2009 Source: Pamela Stanley Lab Wiki, YaleA Simpler Method of Extraction Of DNA From Mouse Tails
http://stanxterm.aecom.yu.edu/wiki/index.php?page=Tail_DNA_extraction_-_simpler_method
Monday,Jul 20,2009 Source: Pamela Stanley Lab Wiki, YaleYolk Sac DNA Preparationor Southern or PCR
http://www.healthsystem.virginia.edu/medicine/inter-dis/transgenic-mouse/YolksacDNA.html
Monday,Jul 20,2009 Source: U. of Virginia Transgenic Mous方法搜索
输入关键字搜索方法
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