X-gal & AP Staining Protocol
Very detailed protocol with background information and several applications.
Monday,Jul 20,2009 Source: Cepko/Tabin LabWhole Mount RNA in situ Hybridization
RNA probe labeling and purification, embryo preparation and more...
Monday,Jul 20,2009 Source: Nagy Labwhole mount preparation and lacZ staining in the mammary gland
The bacterial beta-galactosidase gene lacZ is frequently used as a reporter gene. The expression of transgenic constructs can be monitored by histochemistry with the chromogenic substrate X-gal. This allows precise cellular localization of gene activity.
Monday,Jul 20,2009 Source: Laboratory of Genetics & PhysiWhole Mount LacZ Staining
http://www.mshri.on.ca/nagy/Protocols/lacZ.htm
Monday,Jul 20,2009 Source: Nagy LabWhole Mount Immunoflurescentstaining
http://www.mshri.on.ca/nagy/Protocols/wmimmun.htm
Monday,Jul 20,2009 Source: Nagy LabWhole Mount Human Placental Alkaline Phosphatase Staining
http://www.mshri.on.ca/nagy/Protocols/APwmstain.htm
Monday,Jul 20,2009 Source: Nagy LabTail Preps: DNA Isolation From Mouse Tails Without Phenol
http://hedricklab.ucsd.edu/Protocol/TailPreps.html
Monday,Jul 20,2009 Source: Hedrick Lab, UCSD Cancer CenteRNA Whole Mount In Situ Hybridization
http://axon.med.harvard.edu/~cepko/protocol/ctlab/ish.ct.htm
Monday,Jul 20,2009 Source: Cepko/Tabin LabPreparation of Mouse Tail DNA for Dot Blots or PCR
These procedures were originally devised in Richard Palmiter's lab for use with tail dots (DNA spotted onto a nitrocellulose filter and probed for a transgene; quicker than doing southerns), and subsequently modified for PCR preps.
Monday,Jul 20,2009 Source: Eric MercerPreparation of Copy Standards for PCR Genotyping
The purpose of spiking tail DNA with a known amount of transgene DNA is to produce copy standards. These are used to verify the sensitivty of your PCR genotyping assay and to establish transgene copy number in Southern blot analysis.
Monday,Jul 20,2009 Source: University of Michigan Transge方法搜索
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