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    Xenopus Microinjection Protocol

    http://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/inject.html

    Sunday,Jul 12,2009        Source: Harland Lab, UC Berkely

    Xenopus Tropicalis Sperm Freezing

    X. tropicalis sperm can be frozen for long term storage of lines and then later thawed to recover the line.

    Sunday,Jul 12,2009        Source: Harland Lab, UC Berkely

    X. tropicalis Mating Protocol

    This protocol is designed to produce the maximum number of offspring from a natural male/female mating. It uses artificial stimulation by HCG to induce maturation of gametes and mating behaviors.

    Sunday,Jul 12,2009        Source: Grainger Lab, University of Vi

    Squeezing Females for Eggs

    Squeezing females to get eggs for IVF

    Sunday,Jul 12,2009        Source: Harland Lab, UC Berkely

    Sea Urchin Gamete collection

    http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Urchin/Urchin_fert.html

    Sunday,Jul 12,2009        Source: Experiments in Developmental B

    Sea Urchin Fertilization

    http://tropicalis.berkeley.edu/home/obtaining_embryos/natural_mating.html

    Sunday,Jul 12,2009        Source: Experiments in Developmental B

    Natural Matings

    how to setup matings between males and femals to harvest eggs

    Sunday,Jul 12,2009        Source: Harland Lab, UC Berkely

    Isolating Frog Testis

    Isolating testis for in vitro fertilization (IVF) of eggs

    Sunday,Jul 12,2009        Source: Harland Lab, UC Berkely

    In Vitro Fertilization of X. tropicalis

    http://tropicalis.berkeley.edu/home/obtaining_embryos/IVF.html

    Sunday,Jul 12,2009        Source: Harland Lab, UC Berkely

    hCG Injection

    Injecting trops with hCG to induce ovulation

    Sunday,Jul 12,2009        Source: Harland Lab, UC Berkely
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