Xenopus Microinjection Protocol
http://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/inject.html
Sunday,Jul 12,2009 Source: Harland Lab, UC Berkelyhttp://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/inject.html
Sunday,Jul 12,2009 Source: Harland Lab, UC BerkelyX. tropicalis sperm can be frozen for long term storage of lines and then later thawed to recover the line.
Sunday,Jul 12,2009 Source: Harland Lab, UC BerkelyThis protocol is designed to produce the maximum number of offspring from a natural male/female mating. It uses artificial stimulation by HCG to induce maturation of gametes and mating behaviors.
Sunday,Jul 12,2009 Source: Grainger Lab, University of ViSqueezing females to get eggs for IVF
Sunday,Jul 12,2009 Source: Harland Lab, UC Berkelyhttp://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Urchin/Urchin_fert.html
Sunday,Jul 12,2009 Source: Experiments in Developmental Bhttp://tropicalis.berkeley.edu/home/obtaining_embryos/natural_mating.html
Sunday,Jul 12,2009 Source: Experiments in Developmental Bhow to setup matings between males and femals to harvest eggs
Sunday,Jul 12,2009 Source: Harland Lab, UC BerkelyIsolating testis for in vitro fertilization (IVF) of eggs
Sunday,Jul 12,2009 Source: Harland Lab, UC Berkelyhttp://tropicalis.berkeley.edu/home/obtaining_embryos/IVF.html
Sunday,Jul 12,2009 Source: Harland Lab, UC BerkelyInjecting trops with hCG to induce ovulation
Sunday,Jul 12,2009 Source: Harland Lab, UC Berkely