There are two kinds of dishes used to grow tissue culture cells.Those that are designed for adherent cells have been treated chemically to promote cell adhesion and are called tissue culture dishes. Regular petri dishes have not been treated. Tissue culture dishes are much more expensive than regular petri dishes.
Wednesday,Jul 15,2009 Source: Sefton Lab, Salk InstituteThis technical note is a compendium of techniques, collected from Corning Cell Culture facilities and customers, to reduce the occurrence of irregular patterns of cell adhesion or edge effect in microplates.
Wednesday,Jul 15,2009 Source: CorningThis 4-page guide gives the recommended medium volumes, approximate growth surface areas and average cell yields for Corning plastic culture vessels.
Wednesday,Jul 15,2009 Source: CorningClean and acid wash of coverslips
Wednesday,Jul 15,2009 Source: Howe Lab, University of Vermon3-page protocol that describes how to isolate and develop a population of cells (a clone) that are the descendants of a single cell.
Wednesday,Jul 15,2009 Source: Corninghttp://pingu.salk.edu/~sefton/Hyper_protocols/trypsin.html
Wednesday,Jul 15,2009 Source: Sefton Lab, Salk Institutehttp://www.musc.edu/BCMB/ceramide/protocols/0005.html
Wednesday,Jul 15,2009 Source: Suprya JayadevIt describes in great detail how to pick up clones using a cloning cylinder, along with general transfection procedures.
Wednesday,Jul 15,2009 Source: Cell Biology Lab, School of AnFollowing propagation to 1 X 108 cells, lymphoblastoid cells are conveniently stored at -80 degrees C to preserve the high molecular weight DNA in the cells until the DNA is purified. This procedure describes the steps required to harvest and freeze the cells for long term storage.
Wednesday,Jul 15,2009 Source: Donis-Keller labThis 3-page protocol describes a simple approach using a 96 well plate to isolate colonies derived from single cells.
Wednesday,Jul 15,2009 Source: Corning
