Cell Adherence Assay
Proteins are coated on microtiter plates and cells are added; after the nonbound cells are washed away...
Tuesday,Jul 14,2009 Source: Laurie Lab, University of VirgCarbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates
his procedure allows detection of specific cell adhesion to glycolipids resolved on TLC plates. The steps include: (1) HPTLC of glycoconjugates, (2) coating the chromatogram with polymer, (3) preblocking the chromatogram to reduce non-specific cell binding, (4) mounting the chromatogram in the acrylic chamber, (5) adding labeled cells, (6) incubating, (7) removal of non-adherent cells by centrifugation, and (8) fixation of adherent cells for detection.
Tuesday,Jul 14,2009 Source: GlycoteckAssay for Adhesion and Agar Invasion in S. cerevisiae
Involves growing cells in liquid media and plating on differential nutrient conditions for growth of large spots, which is then washed with water to assess adhesion and rub cells completely off the agar surface to assess invasion into the agar.
Tuesday,Jul 14,2009 Source: Journal of Visualized Experime3D Assay in Matrigel
http://www.lbl.gov/LBL-Programs/lifesciences/BissellLab/labprotocols/ehs3dassay.htm
Tuesday,Jul 14,2009 Source: Bissell Lab, Berkely LabChemotaxis Assay
The purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cell type. Chemotaxis is the ability of a protein to direct the migration of a specific cell. This assay is based on the premise of creating a gradient of the chemotactic agent and allowing cells to migrate through a membrane towards the chemotactic agent. If the agent is not chemotactic for the cell, then the majority of the cells will re
Tuesday,Jul 14,2009 Source: Springer Lab, Harvard UniversiCell Migration Assay
http://bts.ucsf.edu/chen/Protocol/Migration%20Assay%20Protocol.pdf
Tuesday,Jul 14,2009 Source: Chen Lab, UCSF方法搜索
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