氨基酸和蛋白质

酶分析

脂质

进入实验方法社区讨论

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V8 partial proteolytic mapping

Partial proteolysis with Staph. aureus V8 protease http://pingu.salk.edu/~sefton/Hyper_protocols/Cleveland.html

Saturday,Jul 11,2009 Source: Sefton Lab, Salk Institute

Peptide Mapping Analysis Techniques

In-gel digestion procedure for peptide mass mapping of picomole to subpicomole quantities of protein derived from coomassie- or silver stained polyacrylamide gels. First, SDS is removed from the gel prior to digestion, in several ammoniumca

Saturday,Jul 11,2009 Source: R. Aebersold, Vrije Universite

Peptide map prediction

The programs Mobility and Pepsort in GCG package can be used together to predict and plot the theoretical mobilities of peptides in two dimensional thin layer analysis. The plots show electrophoresis in the first dimension and ascending chr

Saturday,Jul 11,2009 Source: Sefton Lab, Salk Institute

CNBr mapping

Kunxin Luo/James Hardwick CNBr Cleavage Procedure http://pingu.salk.edu/~sefton/Hyper_protocols/CNBR.html

Saturday,Jul 11,2009 Source: Sefton Lab, Salk Institute

Two dimensional peptide mapping

This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years http://pingu.salk.edu/~sefton/Hyper_protocols/Tryptic.html

Saturday,Jul 11,2009 Source: Sefton Lab, Salk Institute

Protein Crystallization

http://www.bio.davidson.edu/courses/MolBio/MolStudents/spring2003/Kogoy/protein.html

Saturday,Jul 11,2009 Source: Department of Biology, Davidso

Crystallization Trials

http://www.proweb.org/kinesin/Methods/Xtal_trials.html

Saturday,Jul 11,2009 Source: Kinesin Home Page

Crystallization of Kinesin Family Motor Proteins

Crystallization conditions for kinesin motor protein constructs http://www.proweb.org/kinesin/Methods/Xtal_conditions.html

Saturday,Jul 11,2009 Source: Kinesin Home Page

Radioiodination of protein

Radioiodination of proteins in solution and radioiodination of cell surface proteins http://labs.idi.harvard.edu/springer/protocols/jun_radioiodination.html

Saturday,Jul 11,2009 Source: Springer Lab, Harvard Universi

Purification of acidic phosphatase from mustard seedlings

Phosphate esters are widely distributed in any organism. Nucleic acids, metabolic intermediates like glucose-6-phosphate, energy-rich substrates (AMP, creatine phosphate) are some obvious examples. While many metabolic intermediates are act

Saturday,Jul 11,2009 Source: Dr. Norbert H. Haunerland, Sim

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