Protein arginine methylation
Step by step protocol with introduction to the background information about the method. http://www.methods.info/Methods/Protein_modifications/arg_methylation.pdf
Saturday,Jul 11,2009 Source: Methods.infoPreparing a Selenomethionyl Protein
The protocol describes how to prepare selenomethionyl (Se-Met) protein using a regular E.coli strain. Selenium can be used for phase determination in multi-wavelength anomalous diffraction (MAD) method. Se-Met can often replace methionine r
Saturday,Jul 11,2009 Source: Springer Lab, Harvard MedicalPhosphoamino acid analysis
Mark Kamps's method http://pingu.salk.edu/~sefton/Hyper_protocols/Paa.html
Saturday,Jul 11,2009 Source: Sefton Lab, Salk InstitutePermeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
This is a fast, efficient and reproducible recovery procedure for periplasmic L-asparaginase from distinctly related gram-negative bacteria, Escherichia coli, Enterobacter aerogenes and Pseudomonas aeruginosa. As the method uses inexpensive
Saturday,Jul 11,2009 Source: Hikmet Geckil's LabBiosynthetic labeling
http://pingu.salk.edu/~sefton/Hyper_protocols/biosyn.html
Saturday,Jul 11,2009 Source: Sefton Lab, Salk InstituteAngiotensin Protein Kinase Assay
This procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. http://pingu.salk.edu/~sefton/Hyper_protocols/angio.html
Saturday,Jul 11,2009 Source: Bart's Cookbook, The Salk InstAmicon Stirred Ultrafiltration Cells
For protein concentration, gas pressure is applied directly to ultrafiltration cell. Solutes above the membrane's molecular weight (MW) cut-off are retained in cell, while water and solutes below the cut-off pass into the filtrate and out o
Saturday,Jul 11,2009 Source: Sosnick Lab, University of ChiAcetylation (or Succinylation) of Amino Groups on Proteins
Derivitization of amino groups to remove the positive charge characteristics of the protein, for example to determine the importance of amino groups to a protein's function. http://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=6
Saturday,Jul 11,2009 Source: ancock Laboratory Methods, DepBasic Protein Chemistry Techniques
Basic techniques for protein chemistry including Coomassie Blue Stain and destain, dialysis tube preparation and more. http://www.bio.unc.edu/faculty/salmon/lab/protocolsbasicproteinchemistry.html
Saturday,Jul 11,2009 Source: Salmon Lab, University of Nort方法搜索
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