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Nucleic Acids
丛书 Methods in Molecular Biology
ISSN 1064-3745 (Print) 1940-6029 (Online)
学科 Life Sciences and Biochemistry, general
卷 Volume 2
学科 Life Sciences and Biochemistry, general
出版社 Humana Press
DOI 10.1385/0896030644
版权 1985
ISBN 978-0-89603-064-0 (Print) 978-1-59259-489-4 (Online)
学科分类 生物医学和生命科学
学科 Life Sciences and Biochemistry, general
SpringerLink Date 2008年2月2日
Agarose Gel Electrophoresis of DNA Stephen A. Boffey; 43-50
Autoradiography of Gels Containing 32P Verena D. Huebner; Harry R. Matthews; 51-53
Bacterial Transformation Elliot B. Gingold; 237-240
Bacterial Transformation (Kushner Method) J. W. Dale; P. J. Greenaway; 241-244
Chemical Cleavage (Maxam and Gilbert) Method for DNA Sequence Determination Wim Gaastra; 333-341
DABA Fluorescence Assay for Submicrogram Amounts of DNA Theodore Gurney; Elizabeth G. Gurney; 5月11日
Detection of Specific DNA Sequences—The Southern Transfer C.G.P. Mathew; 55-66
DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts Jytte Josephsen; Wim Gaastra; 131-135
DNA Sequence Determination Using Dideoxy Analogs M. J. Owen; 351-366
DNA Transformation of Mammalian Cells Jeffrey W. Pollard; Yunus Luqmani; Alan Bateson; Kokila Chotai; 321-332
Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions R. McGookin; 93-100
Gel Electrophoretic Analysis of DNA Sequencing Products Wim Gaastra; 343-350
Identification of Recombinant Phages by Plaque Hybridization J. W. Dale; P. J. Greenaway; 285-288
Identification of Recombinant Plasmids by In Situ Colony Hybridization J. W. Dale; P. J.; 277-283
Immunological Detection of Gene Expression in Recombinant Clones J. W. Dale; P. J. Greenaway; 293-300
Immunoprecipitation of in Vitro Translation Products with Protein A Bound to Sepharose C. L. Olliver; C. D. Boyd; 157-160
In Vitro Continuation of RNA Synthesis Initiated in Vivo Theodore Gurney; 161-168
In Vitro Packaging of DNA J. W. Dale; P. J. Greenaway; 245-250
In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System C. L. Oliver; C. D. Boyd; 145-155
In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System C. L. Olliver; A. Grobler-Rabie; C. D. Boyd; 137-144
Ligation of DNA with T4 DNA Ligase Wim Gaastra; Kirsten Hansen; 225-230
Messenger RNA Fractionation on Neutral Sucrose Gradients R. McGookin; 121-124
Molecular Cloning in Bacteriophage Lambda and in Cosmids Claus Christiansen; 313-319
One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography Theodore Gurney; 77-92
Plasmid DNA Isolation (Sheared Lysate Method) J. W. Dale; P. J. Greenaway; 185-189
Plasmid DNA Isolation by the Cleared Lysate Method Stephen A. Boffey; 177-183
Plasmid Screening Using Single Colony Lysates J. W. Dale; P. J. Greenaway; 289-292
Preparation and Assay of Phage Lambda J. W. Dale; P. J. Greenaway; 201-209
Preparation of Chromosomal DNA from E. coli J. W. Dale; P. J. Greenaway; 197-200
Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids Theodore Gurney; 35-42
Preparation of Phage Lambda DNA J. W. Dale; P. J. Greenaway; 211-215
Preparation of “RNase-Free” DNase by Alkylation Theodore Gurney; Elizabeth G. Gurney; 13-20
Radiolabeling of DNA by Nick Translation C. G. P. Mathew; 257-261
Radiolabeling of DNA Using Polynucleotide Kinase Wim Gaastra; Jytte Josephsen; 263-268
Radiolabeling of DNA with 3′ Terminal Transferase Wim Gaastra; Per Klemm; 269-271
Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase Wim Gaastra; Jytte Josephsen; 273-276
RNA Extraction by the Guanidine Thiocyanate Procedure R. McGookin; 113-116
RNA Extraction by the Proteinase K Method R. McGookin; 109-112
Small-Scale Plasmid DNA Preparation J. W. Dale; P. J. Greenaway; 191-195
Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA R. McGookin; 169-176
The Burton Assay for DNA Jaap H. Waterborg; Harry R. Matthews; 1月3日
The Estimation of mRNA Content by Poly(U) Hybridization Robert J. Slater; 125-130
The Extraction and Isolation of DNA from Gels Wim Gaastra; Per Linå Jørgensen; 67-76
The Extraction of Total RNA by the Detergent and Phenol Method Robert J. Slater; 101-108
The Identification of Gene Products in Minicells and Maxicells Wim Gaastra; Per Klemm; 309-312
The Isolation of High Molecular Weight Eukaryotic DNA C. G. P. Mathew; 31-34
The Isolation of Maxicells Wim Gaastra; Per Klemm; 305-308
The Isolation of Minicells Wim Gaastra; Per Klemm; 301-304
The Isolation of Satellite DNA by Density Gradient Centrifugation Craig A. Cooney; Harry R. Matthews; 21-29
The Purification of Poly(A)-Containing RNA by Affinity Chromatography Robert J. Slater; 117-120
The Use of Alkaline Phosphatase to Prevent Vector Regeneration J. W. Dale; P. J. Greenaway; 231-236
The Use of Restriction Endonucleases Elliot B. Gingold; 217-223
Yeast Transformation Elliot B. Gingold; 251-255
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